Наборы обратной транскрипции

170-8890

Набор для синтеза кДНК iScript ™, 25 x 20 мкл реакций, включает 5x

Реакционная смесь iScript, фермент iScript, вода без нуклеаз

170-8891

Набор для синтеза кДНК iScript, 100 x 20 мкл реакций

170-8896

Набор для синтеза кДНК iScript ™ Select, 25 x 20 мкл реакций, включает

5x смесь реакции iScript Select, обратная транскриптаза iScript,

смесь олиго (dT), смесь случайных праймеров, ген-специфический праймер (GSP)

раствор энхансера, вода без нуклеаз

170-8897

Набор для синтеза кДНК iScript Select, 100 x 20 мкл реакций

170-8892

Комплект одностадийной RT-PCR iScript с SYBR ® Green, 50 x 50 мкл

реакции, включает в себя обратную транскриптазу iScript для

одностадийная ОТ-ПЦР, 2x реакционная смесь для ОТ-ПЦР SYBR Green,

вода без нуклеаз

170-8893

Набор одностадийной RT-PCR iScript ™ с SYBR® Green,

200 х 50 мкл реакции

170-8894

Комплект одностадийной RT-PCR iScript для зондов, 50 реакций

170-8895

Комплект одностадийной RT-PCR iScript для зондов, 200 реакций

Страница 92

руководство по продукту

© 2006 Bio-Rad Laboratories, Inc. Все права защищены. Руководство по применению ПЦР в реальном времени

88

Реагенты в реальном времени

170-8880

iQ ™ SYBR ® Green Supermix, реакции 100 x 50 мкл

170-8882

iQ SYBR Green Supermix, реакции 500 x 50 мкл

170-8884

iQ SYBR Green Supermix, 1000 х 50 мкл реакций

170-8885

iQ SYBR Green Supermix, реакция 2000 x 50 мкл, флакон 50 мл

170-8860

iQ ™ Supermix, реакции 100 x 50 мкл

170-8862

iQ Supermix, реакции 500 x 50 мкл

170-8864

iQ Supermix, 1000 x 50 мкл реакций

170-8848

iQ ™ Multiplex Powermix, реакции 50 x 50 мкл

170-8849

iQ Multiplex Powermix, реакции 200 x 50 мкл

170-8850

iTaq ™ SYBR ® Green Supermix с ROX, реакции 200 x 50 мкл

170-8851

iTaq SYBR Green Supermix с ROX, реакции 500 x 50 мкл

170-8852

iTaq SYBR Green Supermix с ROX, 1000 х 50 мкл реакций

170-8853

iTaq SYBR Green Supermix с ROX, 2000 х 50 мкл реакций,

Бутылка 50 мл

170-8854

iTaq ™ Supermix с ROX, реакции 200 x 50 мкл

170-8855

iTaq Supermix с ROX, реакции 500 x 50 мкл

170-8856

iTaq Supermix With ROX, 1,000 x 50 µl reactions

170-8857

iTaq Supermix With ROX, 2,000 x 50 µl reactions, 50 ml bottle

170-8858

ROX Passive Reference Dye, 0.5 ml

Conventional PCR Reagents

170-8870

iTaq™ DNA Polymerase, 5 U/µl, includes 250 U polymerase,

1.25 ml 10x PCR buffer, 1.25 ml 50 mM MgCl2 solution

170-8875

iTaq DNA Polymerase, 5 U/µl, includes 5,000 U polymerase,

25 ml 10x PCR buffer, 25 ml 50 mM MgCl2 solution

170-8874

dNTP Mix, 200 µl premixed solution, contains 10 mM each dNTP

(dATP, dCTP, dGTP, dTTP)

Page 93

product guide

Plastics

MSB-1001

Microseal 'B' Seals, clear adhesive, 100

223-9441

96-Well 0.2 ml Thin-Wall PCR Plates, 25

MLL-4851

Multiplate™ Low-Profile 48-Well Unskirted PCR Plates, 8 x 6

format, white, thin-wall polypropylene, 50

MLL-4801

Multiplate Low-Profile 48-Well Unskirted PCR Plates, 8 x 6 format,

natural color, thin-wall polypropylene, 50

Software

170-8734

Beacon Designer Probe/Primer Design Software, includes

CD-ROM, quick guide, instructions

170-9753

iQ5 Optical System Software, version 2.0

170-9753SE01 iQ5 Optical System Software, Security Edition, single-seat license

170-9753SE05 iQ5 Optical System Software, Security Edition, 5-seat license

170-9753SE10 iQ5 Optical System Software, Security Edition, 10-seat license

© 2006 Bio-Rad Laboratories, Inc. All rights reserved. Real-Time PCR Applications Guide

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references and legal notices

© 2006 Bio-Rad Laboratories, Inc. All rights reserved. Real-Time PCR Applications Guide

90

References

Mathews DH et al., Expanded sequence dependence of thermodynamic parameters provides robust

prediction of RNA secondary structure, J Mol Biol 288, 910–940 (1999)

Rozen S and Skaletsky HJ, Primer3 on the WWW for general users and for biologist programmers, pp

365–386 in Bioinformatics Methods and Protocols: Methods in Molecular Biology (Krawetz S and Misener

S, eds), Humana Press, Totowa, NJ (2000); source code available at http://fokker.wi.mit.edu/primer3/

Zuker M et al., Algorithms and thermodynamics for RNA secondary structure prediction: a practical guide,

pp 11–43 in RNA Biochemistry and Biotechnology (Barciszewski J and Clark BFC, eds), Kluwer Academic

Publishers, Boston (1999)

Legal Notices

Amplifluor and UniPrimer are trademarks of Millipore Corporation. AmpliTaq, AmpliTaq Gold, and TaqMan

are trademarks of Roche Molecular Systems, Inc. BD QZyme is a trademark of Becton, Dickinson and

Company. Beacon Designer is a trademark of PREMIER Biosoft International. BHQ and Black Hole

Quencher are trademarks of Biosearch Technologies, Inc. Cy is a trademark of GE Healthcare. DNeasy is

a trademark of Qiagen GmbH Corporation. DyNAzyme is a trademark of Finnzymes Oy. Eclipse is a

trademark of Epoch Biosciences, Inc. Excel is a trademark of Microsoft Corporation. Hoechst is a

trademark of Hoechst Aktiengesellschaft. LUX is a trademark of Invitrogen Corporation. Oligo is a

trademark of Molecular Biology Insights, Inc. PicoGreen, SYBR, and Texas Red are trademarks of

Molecular Probes, Inc. Roundup Ready is a trademark of Monsanto Company. Scorpions is a trademark

of DxS Limited. VIC is a trademark of Applera Corporation.

Molecular beacons and their use are licensed under patents owned by The Public Health Research

Institute of the City of New York, Inc.

Primer3 includes software developed by the Whitehead Institute for Biomedical Research.

Bio-Rad Laboratories, Inc. is licensed by Molecular Probes, Inc. to sell reagents containing SYBR Green I

for use in real-time PCR, for research purposes only.

Notice regarding Bio-Rad thermal cyclers and real-time systems.

Purchase of this instrument conveys a limited non-transferable immunity from suit for the purchaser’s own

internal research and development and for use in applied fields other than Human In Vitro Diagnostics

under one or more of U.S. Patents Nos. 5,656,493, 5,333,675, 5,475,610 (claims 1, 44, 158, 160–163

and 167 only), and 6,703,236 (claims 1–7 only), or corresponding claims in their non-U.S. counterparts,

owned by Applera Corporation. No right is conveyed expressly, by implication or by estoppel under any

other patent claim, such as claims to apparatus, reagents, kits, or methods such as 5' nuclease methods.

Further information on purchasing licenses may be obtained by contacting the Director of Licensing,

Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Bio-Rad's real-time thermal cyclers are licensed real-time thermal cyclers under Applera's United States

Patent No. 6,814,934 B1 for use in research and for all other fields except the fields of human diagnostics

and veterinary diagnostics.

Page 95

notes

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notes

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Исходный текст

a single reaction, the β-actin gene and each of the three target genes, ODC, AZI,

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