Image S6: irregularly contracted cells

 

Unstable haemoglobin

 

J. Burthem, M. Brereton

 

Image S7: schistocytes

Thrombotic thrombocytopenic purpura – a wide range of fragmented red cells with polychromatic cells and other damaged cells. Platelets are absent from the film

 

J. Burthem, M. Brereton

 

Image S8: sickle cells

 

Sickle cell disease – typical forms together with other features, notably polychromasia, target cells, and a spherocytic cell.

 

J. Burthem, M. Brereton

3

 

Supplementary images: ICSH Recommendations for Peripheral Blood Cell Morphology

 

Standardization and Grading

 

 

Image S9: spherocytes

 

Autoimmune haemolytic anaemia – typical round, dense red cells

 

J. Burthem, M. Brereton

 

 

Image S10: stomatocytes

 

Hereditary stomatocytosis

 

J. Burthem, M. Brereton

 

 

Image S11: target cells

 

Haemoglobin C disease – target cells in association with irregularly contracted cells

 

J. Burthem, M. Brereton

 

Image S12: tear drop cells

 

Myelofibrosis

 

J. Burthem, M. Brereton

4

 

Supplementary images: ICSH Recommendations for Peripheral Blood Cell Morphology

 

Standardization and Grading

 

Image S13: basophilic stippling

 

Myelofibrosis - two stippled cells, one in teardrop form

 

J. Burthem, M. Brereton

 

Image S14: Howell-Jolly bodies

Auto-splenectomised patient with sickle cell disease

 

J. Burthem, M. Brereton

 

 

Image S15: Pappenheimer bodies

 

Sideroblastic anaemia – red cells contain small basophilic inclusions of variable size and shape in a limited cytoplasmic area

 

G. Rozenberg

 

(Copyright: Microscopic haematology: a practical guide for the laboratory 3e (c) 2011, Sydney, Elsevier Australia)

 

 

Image S16: nucleated red blood cell

 

Myelofibrosis – a typical late stage nucleated red cell in circulation

 

J. Burthem, M. Brereton

5

 

Supplementary images: ICSH Recommendations for Peripheral Blood Cell Morphology

 

Standardization and Grading

 

 

Image S17: large granular lymphocyte

 

Large granular lymphocyte from a normal individual

 

J. Burthem, M. Brereton

 

Image S18: Auer rods

 

AML – two blast cells containing relatively blunt-ended, single and multiple Auer rods

 

J. Burthem, M. Brereton

 

Image S19: hypergranulation

(neutrophils)

 

Hypergranular neutrophils post G-CSF treatment

 

J. Burthem, M. Brereton

 

 

Image S20: hypogranulation

 

(neutrophils)

 

Myelodysplasia – hypogranular neutrophils.

 

Note also the atypical nuclear forms

 

G. Rozenberg

 

(Copyright: Microscopic haematology: a practical guide for the laboratory 3e (c) 2011, Sydney, Elsevier Australia)

6

 

Supplementary images: ICSH Recommendations for Peripheral Blood Cell Morphology

 

Standardization and Grading