Image S21: Pelger Huet neutrophils

 

Pelger Huet anomaly – classic bi-lobed cells with dense chromatin condensation but normal granulation

 

J. Burthem, M. Brereton

 

Image S22: leukaemic myeloblasts

 

Acute myeloid leukaemia (AML) – hypogranular primitive blast cells

 

J. Burthem, M. Brereton

 

 

Image S23: abnormal promyelocytes in APL (1)

 

APML – two hypergranular promyelocytes

 

J. Burthem, M. Brereton

 

Image S24: abnormal promyelocytes in APL (2)

Abnormal promyelocyte containing multiple Auer rods (faggot cell)

 

G. Rozenberg

 

(Copyright: Microscopic haematology: a practical guide for the laboratory 3e (c) 2011, Sydney, Elsevier Australia)

7

 

Supplementary images: ICSH Recommendations for Peripheral Blood Cell Morphology

 

Standardization and Grading

 

 

Image S25: monoblasts

 

Acute monoblastic leukaemia – monoblasts and promonocytes

 

J. Burthem, M. Brereton

 

Image S26: abnormal promonocytes

Chronic myelomonocytic leukaemia (CMML)

 

G. Zini

 

Image S27: reactive lymphocytes

 

Infectious mononucleosis – typical reactive lymphocytes with flowing basophilic cytoplasm

 

J. Burthem, M. Brereton

 

 

Image S28: hairy cells

 

Hairy cell leukaemia

 

J. Burthem, M. Brereton

8

 

Supplementary images: ICSH Recommendations for Peripheral Blood Cell Morphology

 

Standardization and Grading

 

 

Image S29: follicular lymphoma cells

 

Circulating follicular lymphoma cells – note the small cells with cleaved nuclei and sparse cytoplasm

 

J. Burthem, M. Brereton

 

Image S30: plasma cells

 

Plasma cell leukaemia. Note also the background protein staining and the associated red cell rouleaux. Note that one plasma cell has features of immaturity and may be regarded as a plasmablast.

 

J. Burthem, M. Brereton

 

 

Image S31: prolymphocytic leukaemia cells

B-Prolymphocytic leukaemia

 

J. Burthem, M. Brereton

 

Image S32: chronic lymphocytic leukaemia cells

 

Typical CLL lymphocytes with a smudge cell

 

J. Burthem, M. Brereton

9

 

Supplementary images: ICSH Recommendations for Peripheral Blood Cell Morphology

 

Standardization and Grading

 

 

Image S33: giant platelets

 

Myelofibrosis – some large and giant abnormally granulated platelets and a micromegakaryocyte

 

J. Burthem, M. Brereton

 

Image S34: hypogranular platelets

 

Myelofibrosis – variable platelet appearance with normal and abnormally granulated platelets together with some large hypogranular forms

 

J. Burthem, M. Brereton

 

 

Image S35: micromegakaryocytes

 

Myelofibrosis – note the typical ‘granulated’ platelet cytoplasm. A bare megakaryocyte nucleus is also present

 

J. Burthem, M. Brereton